Construct: shRNA TRCN0000009113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000870.2-1661s1c1
DNA Barcode:
CGGTCACTGATGTCTCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTR4 (3360)
Gene Description:
5-hydroxytryptamine receptor 4
Transcript:
RefSeq NM_000870.2 (NON-CURRENT)
Match location:
Position 1661 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCACTGATGTCTCCAAAT
Hairpin Sequence:
5'-CCGG-CGGTCACTGATGTCTCCAAAT-CTCGAG-ATTTGGAGACATCAGTGACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCACTGATGTCTCCAAATCTCGAGATTTGGAGACATCAGTGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCACTGATGTCTCCAAATCTCGAGATTTGGAGACATCAGTGACCG-3'

Other clones with same target sequence:

(none)