Construct: shRNA TRCN0000014141
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_177437.1-564s1c1
- DNA Barcode:
- CTGTGAGAAATTCTATCTCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAS2R60 (338398)
- Gene Description:
- taste 2 receptor member 60
- Transcript:
- RefSeq NM_177437.1 (CURRENT)
- Match location:
- Position 564 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000014141)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGTGAGAAATTCTATCTCTT
- Hairpin Sequence:
-
5'-CCGG-CTGTGAGAAATTCTATCTCTT-CTCGAG-AAGAGATAGAATTTCTCACAG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGTGAGAAATTCTATCTCTTCTCGAGAAGAGATAGAATTTCTCACAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGTGAGAAATTCTATCTCTTCTCGAGAAGAGATAGAATTTCTCACAG-3'
Other clones with same target sequence:
(none)
