Construct: shRNA TRCN0000015071

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005068.2-1200s1c1
DNA Barcode:
CCTCACAGACACAGAATACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIM1 (6492)
Gene Description:
SIM bHLH transcription factor 1
Transcript:
RefSeq NM_005068.2 (CURRENT)
Match location:
Position 1200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015071)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACAGACACAGAATACAA
Hairpin Sequence:
5'-CCGG-CCTCACAGACACAGAATACAA-CTCGAG-TTGTATTCTGTGTCTGTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACAGACACAGAATACAACTCGAGTTGTATTCTGTGTCTGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACAGACACAGAATACAACTCGAGTTGTATTCTGTGTCTGTGAGG-3'

Other clones with same target sequence:

(none)