Construct: shRNA TRCN0000017423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007017.2-1324s1c1
DNA Barcode:
CCCTTCAGTAAGGACAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOX30 (11063)
Gene Description:
SRY-box transcription factor 30
Transcript:
RefSeq NM_007017.2 (CURRENT)
Match location:
Position 1324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCAGTAAGGACAGAAAT
Hairpin Sequence:
5'-CCGG-CCCTTCAGTAAGGACAGAAAT-CTCGAG-ATTTCTGTCCTTACTGAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCAGTAAGGACAGAAATCTCGAGATTTCTGTCCTTACTGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCAGTAAGGACAGAAATCTCGAGATTTCTGTCCTTACTGAAGGG-3'

Other clones with same target sequence:

(none)