Construct: shRNA TRCN0000019272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003146.2-1375s1c1
DNA Barcode:
CGCTTCGATGAGATCTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSRP1 (6749)
Gene Description:
structure specific recognition protein 1
Transcript:
RefSeq NM_003146.2 (NON-CURRENT)
Match location:
Position 1375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTCGATGAGATCTCCTTT
Hairpin Sequence:
5'-CCGG-CGCTTCGATGAGATCTCCTTT-CTCGAG-AAAGGAGATCTCATCGAAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTCGATGAGATCTCCTTTCTCGAGAAAGGAGATCTCATCGAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTCGATGAGATCTCCTTTCTCGAGAAAGGAGATCTCATCGAAGCG-3'

Other clones with same target sequence:

  1. TRCN0000343894