Construct: shRNA TRCN0000021444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_208545.4-1474s1c1
DNA Barcode:
CCTGAGACATTCAGTTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC283155 (283155)
Gene Description:
similar to ALK-3
Transcript:
RefSeq XM_208545.4 (NON-CURRENT)
Match location:
Position 1474 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGACATTCAGTTCCTTT
Hairpin Sequence:
5'-CCGG-CCTGAGACATTCAGTTCCTTT-CTCGAG-AAAGGAACTGAATGTCTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGACATTCAGTTCCTTTCTCGAGAAAGGAACTGAATGTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGACATTCAGTTCCTTTCTCGAGAAAGGAACTGAATGTCTCAGG-3'

Other clones with same target sequence:

(none)