Construct: shRNA TRCN0000021448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_208545.4-278s1c1
DNA Barcode:
GCTGGTTTCGAGAAACAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC283155 (283155)
Gene Description:
similar to ALK-3
Transcript:
RefSeq XM_208545.4 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTTCGAGAAACAGAAA
Hairpin Sequence:
5'-CCGG-GCTGGTTTCGAGAAACAGAAA-CTCGAG-TTTCTGTTTCTCGAAACCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTTCGAGAAACAGAAACTCGAGTTTCTGTTTCTCGAAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTTCGAGAAACAGAAACTCGAGTTTCTGTTTCTCGAAACCAGC-3'

Other clones with same target sequence:

(none)