Construct: shRNA TRCN0000021477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025144.2-1319s1c1
DNA Barcode:
GCCCAGGTGAAGGAACATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALPK1 (80216)
Gene Description:
alpha kinase 1
Transcript:
RefSeq NM_025144.2 (NON-CURRENT)
Match location:
Position 1319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGGTGAAGGAACATTTA
Hairpin Sequence:
5'-CCGG-GCCCAGGTGAAGGAACATTTA-CTCGAG-TAAATGTTCCTTCACCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGGTGAAGGAACATTTACTCGAGTAAATGTTCCTTCACCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGGTGAAGGAACATTTACTCGAGTAAATGTTCCTTCACCTGGGC-3'

Other clones with same target sequence:

(none)