Construct: shRNA TRCN0000021478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025144.2-3416s1c1
DNA Barcode:
CCATCCACAATACTACTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALPK1 (80216)
Gene Description:
alpha kinase 1
Transcript:
RefSeq NM_025144.2 (NON-CURRENT)
Match location:
Position 3416 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCACAATACTACTGATT
Hairpin Sequence:
5'-CCGG-CCATCCACAATACTACTGATT-CTCGAG-AATCAGTAGTATTGTGGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCACAATACTACTGATTCTCGAGAATCAGTAGTATTGTGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCACAATACTACTGATTCTCGAGAATCAGTAGTATTGTGGATGG-3'

Other clones with same target sequence:

(none)