Construct: shRNA TRCN0000025172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172858.1-1734s1c1
DNA Barcode:
CCTGCAAACCAGTTCTCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pak7 (241656)
Gene Description:
p21 protein (Cdc42/Rac)-activated kinase 7
Transcript:
RefSeq NM_172858.1 (NON-CURRENT)
Match location:
Position 1734 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAAACCAGTTCTCAGTA
Hairpin Sequence:
5'-CCGG-CCTGCAAACCAGTTCTCAGTA-CTCGAG-TACTGAGAACTGGTTTGCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAAACCAGTTCTCAGTACTCGAGTACTGAGAACTGGTTTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAAACCAGTTCTCAGTACTCGAGTACTGAGAACTGGTTTGCAGG-3'

Other clones with same target sequence:

(none)