Construct: shRNA TRCN0000025524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010294.1-609s1c1
DNA Barcode:
GCATCCATTGCACCGATGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gk2 (14626)
Gene Description:
glycerol kinase 2
Transcript:
RefSeq NM_010294.1 (CURRENT)
Match location:
Position 609 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCATTGCACCGATGTAA
Hairpin Sequence:
5'-CCGG-GCATCCATTGCACCGATGTAA-CTCGAG-TTACATCGGTGCAATGGATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCATTGCACCGATGTAACTCGAGTTACATCGGTGCAATGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCATTGCACCGATGTAACTCGAGTTACATCGGTGCAATGGATGC-3'

Other clones with same target sequence:

(none)