Construct: shRNA TRCN0000025526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010294.1-1343s1c1
DNA Barcode:
CAGGCAGATATTCTGCACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gk2 (14626)
Gene Description:
glycerol kinase 2
Transcript:
RefSeq NM_010294.1 (CURRENT)
Match location:
Position 1343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCAGATATTCTGCACATT
Hairpin Sequence:
5'-CCGG-CAGGCAGATATTCTGCACATT-CTCGAG-AATGTGCAGAATATCTGCCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCAGATATTCTGCACATTCTCGAGAATGTGCAGAATATCTGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCAGATATTCTGCACATTCTCGAGAATGTGCAGAATATCTGCCTG-3'

Other clones with same target sequence:

(none)