Construct: shRNA TRCN0000025933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008539.3-1774s1c1
DNA Barcode:
TCCTATTTCATCCGTGTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad1 (17125)
Gene Description:
SMAD family member 1
Transcript:
RefSeq NM_008539.3 (CURRENT)
Match location:
Position 1774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTATTTCATCCGTGTCTTA
Hairpin Sequence:
5'-CCGG-TCCTATTTCATCCGTGTCTTA-CTCGAG-TAAGACACGGATGAAATAGGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTATTTCATCCGTGTCTTACTCGAGTAAGACACGGATGAAATAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTATTTCATCCGTGTCTTACTCGAGTAAGACACGGATGAAATAGGA-3'

Other clones with same target sequence:

  1. TRCN0000285960