Construct: shRNA TRCN0000026306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013461.2-1940s1c1
DNA Barcode:
CCTTTCAGAATGTGCTGCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adra1a (11549)
Gene Description:
adrenergic receptor, alpha 1a
Transcript:
RefSeq NM_013461.2 (NON-CURRENT)
Match location:
Position 1940 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCAGAATGTGCTGCGAA
Hairpin Sequence:
5'-CCGG-CCTTTCAGAATGTGCTGCGAA-CTCGAG-TTCGCAGCACATTCTGAAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCAGAATGTGCTGCGAACTCGAGTTCGCAGCACATTCTGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCAGAATGTGCTGCGAACTCGAGTTCGCAGCACATTCTGAAAGG-3'

Other clones with same target sequence:

(none)