Construct: shRNA TRCN0000027381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007551.2-742s1c1
DNA Barcode:
GCTTGTGATGGGATGGTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cxcr5 (12145)
Gene Description:
chemokine (C-X-C motif) receptor 5
Transcript:
RefSeq NM_007551.2 (CURRENT)
Match location:
Position 742 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTGATGGGATGGTGTTA
Hairpin Sequence:
5'-CCGG-GCTTGTGATGGGATGGTGTTA-CTCGAG-TAACACCATCCCATCACAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTGATGGGATGGTGTTACTCGAGTAACACCATCCCATCACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTGATGGGATGGTGTTACTCGAGTAACACCATCCCATCACAAGC-3'

Other clones with same target sequence:

(none)