Construct: shRNA TRCN0000030504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008437.1-1045s1c1
DNA Barcode:
CGGGCCTTGAATAAAGCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Napsa (16541)
Gene Description:
napsin A aspartic peptidase
Transcript:
RefSeq NM_008437.1 (CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCCTTGAATAAAGCCATT
Hairpin Sequence:
5'-CCGG-CGGGCCTTGAATAAAGCCATT-CTCGAG-AATGGCTTTATTCAAGGCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCCTTGAATAAAGCCATTCTCGAGAATGGCTTTATTCAAGGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCCTTGAATAAAGCCATTCTCGAGAATGGCTTTATTCAAGGCCCG-3'

Other clones with same target sequence:

(none)