Construct: shRNA TRCN0000030828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013918.2-286s1c1
DNA Barcode:
GCAGATGCAAATGTAATTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp25 (30940)
Gene Description:
ubiquitin specific peptidase 25
Transcript:
RefSeq NM_013918.2 (CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGCAAATGTAATTGAT
Hairpin Sequence:
5'-CCGG-GCAGATGCAAATGTAATTGAT-CTCGAG-ATCAATTACATTTGCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGCAAATGTAATTGATCTCGAGATCAATTACATTTGCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGCAAATGTAATTGATCTCGAGATCAATTACATTTGCATCTGC-3'

Other clones with same target sequence:

(none)