Transcript: Mouse NM_013918.2

Mus musculus ubiquitin specific peptidase 25 (Usp25), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Usp25 (30940)
Length:
4432
CDS:
28..3195

Additional Resources:

NCBI RefSeq record:
NM_013918.2
NBCI Gene record:
Usp25 (30940)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013918.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000233430 GACGTGAACAATGGCTTAATT pLKO_005 2875 CDS 100% 15.000 21.000 N Usp25 n/a
2 TRCN0000233429 GGAGTGGCATGCAGATTATAA pLKO_005 2622 CDS 100% 15.000 21.000 N Usp25 n/a
3 TRCN0000233431 TAGTATAATGGAACCATATTG pLKO_005 3723 3UTR 100% 13.200 18.480 N Usp25 n/a
4 TRCN0000030824 CGGTGGATGAAGTATAATGAT pLKO.1 1885 CDS 100% 5.625 7.875 N Usp25 n/a
5 TRCN0000233428 CATCGCTGGAGGACGGAAATA pLKO_005 1687 CDS 100% 13.200 9.240 N Usp25 n/a
6 TRCN0000233427 TTATATCTGGACAGGTATATG pLKO_005 1222 CDS 100% 13.200 9.240 N Usp25 n/a
7 TRCN0000030827 CCCAACGATCACTGCAAGAAA pLKO.1 2153 CDS 100% 5.625 3.938 N Usp25 n/a
8 TRCN0000030828 GCAGATGCAAATGTAATTGAT pLKO.1 286 CDS 100% 5.625 3.938 N Usp25 n/a
9 TRCN0000030825 CCTGCTGGTTTAGTGCAGTTA pLKO.1 557 CDS 100% 4.950 3.465 N Usp25 n/a
10 TRCN0000030826 GCTTGATTGTTCTACAGAGAT pLKO.1 3063 CDS 100% 4.950 3.465 N Usp25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013918.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03088 pDONR223 100% 87.8% 93.8% None (many diffs) n/a
2 ccsbBroad304_03088 pLX_304 0% 87.8% 93.8% V5 (many diffs) n/a
3 TRCN0000467162 GTTAGACCGGTCGCTGGTCCGGGT pLX_317 14.4% 87.8% 93.8% V5 (many diffs) n/a
4 ccsbBroadEn_11902 pDONR223 100% 37.8% 40.7% None (many diffs) n/a
5 ccsbBroad304_11902 pLX_304 0% 37.8% 40.7% V5 (many diffs) n/a
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