Construct: shRNA TRCN0000030876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027554.2-2973s1c1
DNA Barcode:
CCCAAATTAGTGCCCTACCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp38 (74841)
Gene Description:
ubiquitin specific peptidase 38
Transcript:
RefSeq NM_027554.2 (CURRENT)
Match location:
Position 2973 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAATTAGTGCCCTACCTA
Hairpin Sequence:
5'-CCGG-CCCAAATTAGTGCCCTACCTA-CTCGAG-TAGGTAGGGCACTAATTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAATTAGTGCCCTACCTACTCGAGTAGGTAGGGCACTAATTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAATTAGTGCCCTACCTACTCGAGTAGGTAGGGCACTAATTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000327301