Construct: shRNA TRCN0000031469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023131.1-780s1c1
DNA Barcode:
CCACTCTTTCTGCAACTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rce1 (19671)
Gene Description:
Ras converting CAAX endopeptidase 1
Transcript:
RefSeq NM_023131.1 (CURRENT)
Match location:
Position 780 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCTTTCTGCAACTACAT
Hairpin Sequence:
5'-CCGG-CCACTCTTTCTGCAACTACAT-CTCGAG-ATGTAGTTGCAGAAAGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCTTTCTGCAACTACATCTCGAGATGTAGTTGCAGAAAGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCTTTCTGCAACTACATCTCGAGATGTAGTTGCAGAAAGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000354157