Construct: shRNA TRCN0000031652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145131.1-2316s1c1
DNA Barcode:
CGGATCAAGAAGTATCTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pitrm1 (69617)
Gene Description:
pitrilysin metallepetidase 1
Transcript:
RefSeq NM_145131.1 (CURRENT)
Match location:
Position 2316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATCAAGAAGTATCTACTA
Hairpin Sequence:
5'-CCGG-CGGATCAAGAAGTATCTACTA-CTCGAG-TAGTAGATACTTCTTGATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATCAAGAAGTATCTACTACTCGAGTAGTAGATACTTCTTGATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATCAAGAAGTATCTACTACTCGAGTAGTAGATACTTCTTGATCCG-3'

Other clones with same target sequence:

  1. TRCN0000324790