Construct: shRNA TRCN0000032731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023047.2-1234s1c1
DNA Barcode:
CTGAACATTGTGGCATCCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpysl5 (65254)
Gene Description:
dihydropyrimidinase-like 5
Transcript:
RefSeq NM_023047.2 (CURRENT)
Match location:
Position 1234 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAACATTGTGGCATCCGAT
Hairpin Sequence:
5'-CCGG-CTGAACATTGTGGCATCCGAT-CTCGAG-ATCGGATGCCACAATGTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAACATTGTGGCATCCGATCTCGAGATCGGATGCCACAATGTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAACATTGTGGCATCCGATCTCGAGATCGGATGCCACAATGTTCAG-3'

Other clones with same target sequence:

(none)