Construct: shRNA TRCN0000034574
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_019078.1-257s1c1
- DNA Barcode:
- AGGACGAATTTGATCGCCTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UGT1A5 (54579)
- Gene Description:
- UDP glucuronosyltransferase family 1 member A5
- Transcript:
- RefSeq NM_019078.1 (CURRENT)
- Match location:
- Position 257 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000034574)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGGACGAATTTGATCGCCTTT
- Hairpin Sequence:
-
5'-CCGG-AGGACGAATTTGATCGCCTTT-CTCGAG-AAAGGCGATCAAATTCGTCCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGGACGAATTTGATCGCCTTTCTCGAGAAAGGCGATCAAATTCGTCCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGGACGAATTTGATCGCCTTTCTCGAGAAAGGCGATCAAATTCGTCCT-3'
Other clones with same target sequence:
(none)
