Construct: shRNA TRCN0000034576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019078.1-672s1c1
DNA Barcode:
CCATGCTGTTTCTGCTCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A5 (54579)
Gene Description:
UDP glucuronosyltransferase family 1 member A5
Transcript:
RefSeq NM_019078.1 (CURRENT)
Match location:
Position 672 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTGTTTCTGCTCCTTA
Hairpin Sequence:
5'-CCGG-CCATGCTGTTTCTGCTCCTTA-CTCGAG-TAAGGAGCAGAAACAGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTGTTTCTGCTCCTTACTCGAGTAAGGAGCAGAAACAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTGTTTCTGCTCCTTACTCGAGTAAGGAGCAGAAACAGCATGG-3'

Other clones with same target sequence:

(none)