Construct: shRNA TRCN0000034734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003642.1-1264s1c1
DNA Barcode:
CGGCGTGTTATTGAACGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HAT1 (8520)
Gene Description:
histone acetyltransferase 1
Transcript:
RefSeq NM_003642.1 (NON-CURRENT)
Match location:
Position 1264 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCGTGTTATTGAACGACTT
Hairpin Sequence:
5'-CCGG-CGGCGTGTTATTGAACGACTT-CTCGAG-AAGTCGTTCAATAACACGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCGTGTTATTGAACGACTTCTCGAGAAGTCGTTCAATAACACGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCGTGTTATTGAACGACTTCTCGAGAAGTCGTTCAATAACACGCCG-3'

Other clones with same target sequence:

  1. TRCN0000289816