Construct: shRNA TRCN0000035274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018973.3-159s1c1
DNA Barcode:
GCAGTGACCATGACGAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPM3 (54344)
Gene Description:
dolichyl-phosphate mannosyltransferase subunit 3, regulatory
Transcript:
RefSeq NM_018973.3 (CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGACCATGACGAAATTA
Hairpin Sequence:
5'-CCGG-GCAGTGACCATGACGAAATTA-CTCGAG-TAATTTCGTCATGGTCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGACCATGACGAAATTACTCGAGTAATTTCGTCATGGTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGACCATGACGAAATTACTCGAGTAATTTCGTCATGGTCACTGC-3'

Other clones with same target sequence:

  1. TRCN0000291736