Construct: shRNA TRCN0000035353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004605.2-362s1c1
DNA Barcode:
GATCGAGATCATCTGCTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SULT2B1 (6820)
Gene Description:
sulfotransferase family 2B member 1
Transcript:
RefSeq NM_004605.2 (CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGAGATCATCTGCTTAAT
Hairpin Sequence:
5'-CCGG-GATCGAGATCATCTGCTTAAT-CTCGAG-ATTAAGCAGATGATCTCGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGAGATCATCTGCTTAATCTCGAGATTAAGCAGATGATCTCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGAGATCATCTGCTTAATCTCGAGATTAAGCAGATGATCTCGATC-3'

Other clones with same target sequence:

  1. TRCN0000333184