Construct: shRNA TRCN0000037202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018771.3-731s1c1
DNA Barcode:
GAACCTCGAAAGGCCTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gipc1 (67903)
Gene Description:
GIPC PDZ domain containing family, member 1
Transcript:
RefSeq NM_018771.3 (CURRENT)
Match location:
Position 731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTCGAAAGGCCTTTGAT
Hairpin Sequence:
5'-CCGG-GAACCTCGAAAGGCCTTTGAT-CTCGAG-ATCAAAGGCCTTTCGAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTCGAAAGGCCTTTGATCTCGAGATCAAAGGCCTTTCGAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTCGAAAGGCCTTTGATCTCGAGATCAAAGGCCTTTCGAGGTTC-3'

Other clones with same target sequence:

  1. TRCN0000308952