Construct: shRNA TRCN0000041019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144860.2-2388s1c1
DNA Barcode:
GCCATAAGTAAGAAACGGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mib1 (225164)
Gene Description:
mindbomb E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_144860.2 (CURRENT)
Match location:
Position 2388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATAAGTAAGAAACGGGAT
Hairpin Sequence:
5'-CCGG-GCCATAAGTAAGAAACGGGAT-CTCGAG-ATCCCGTTTCTTACTTATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATAAGTAAGAAACGGGATCTCGAGATCCCGTTTCTTACTTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATAAGTAAGAAACGGGATCTCGAGATCCCGTTTCTTACTTATGGC-3'

Other clones with same target sequence:

(none)