Construct: shRNA TRCN0000044826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001094.4-1424s1c1
DNA Barcode:
GCCAAGTACCTTGAGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASIC2 (40)
Gene Description:
acid sensing ion channel subunit 2
Transcript:
RefSeq NM_001094.4 (CURRENT)
Match location:
Position 1424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTACCTTGAGAAGAAA
Hairpin Sequence:
5'-CCGG-GCCAAGTACCTTGAGAAGAAA-CTCGAG-TTTCTTCTCAAGGTACTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTACCTTGAGAAGAAACTCGAGTTTCTTCTCAAGGTACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTACCTTGAGAAGAAACTCGAGTTTCTTCTCAAGGTACTTGGC-3'

Other clones with same target sequence:

(none)