Construct: shRNA TRCN0000045800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030783.1-507s1c1
DNA Barcode:
CAAGCTAAAGACGGGCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTDSS2 (81490)
Gene Description:
phosphatidylserine synthase 2
Transcript:
RefSeq NM_030783.1 (NON-CURRENT)
Match location:
Position 507 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCTAAAGACGGGCCATTT
Hairpin Sequence:
5'-CCGG-CAAGCTAAAGACGGGCCATTT-CTCGAG-AAATGGCCCGTCTTTAGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCTAAAGACGGGCCATTTCTCGAGAAATGGCCCGTCTTTAGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCTAAAGACGGGCCATTTCTCGAGAAATGGCCCGTCTTTAGCTTG-3'

Other clones with same target sequence:

  1. TRCN0000229306
  2. TRCN0000350003