Construct: shRNA TRCN0000350003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013782.4-381s21c1
DNA Barcode:
CAAGCTAAAGACGGGCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptdss2 (27388)
Gene Description:
phosphatidylserine synthase 2
Transcript:
RefSeq NM_013782.4 (CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCTAAAGACGGGCCATTT
Hairpin Sequence:
5'-CCGG-CAAGCTAAAGACGGGCCATTT-CTCGAG-AAATGGCCCGTCTTTAGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCTAAAGACGGGCCATTTCTCGAGAAATGGCCCGTCTTTAGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCTAAAGACGGGCCATTTCTCGAGAAATGGCCCGTCTTTAGCTTG-3'

Other clones with same target sequence:

  1. TRCN0000045800
  2. TRCN0000229306