Construct: shRNA TRCN0000046663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020868.1-716s1c1
DNA Barcode:
CGGTGGATCAATGATACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPP10 (57628)
Gene Description:
dipeptidyl peptidase like 10
Transcript:
RefSeq NM_020868.1 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGATCAATGATACAGAT
Hairpin Sequence:
5'-CCGG-CGGTGGATCAATGATACAGAT-CTCGAG-ATCTGTATCATTGATCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGATCAATGATACAGATCTCGAGATCTGTATCATTGATCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGATCAATGATACAGATCTCGAGATCTGTATCATTGATCCACCG-3'

Other clones with same target sequence:

(none)