Construct: shRNA TRCN0000048388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014857.2-1926s1c1
DNA Barcode:
CGAGATATTCATCGTACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RABGAP1L (9910)
Gene Description:
RAB GTPase activating protein 1 like
Transcript:
RefSeq NM_014857.2 (NON-CURRENT)
Match location:
Position 1926 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATATTCATCGTACATTT
Hairpin Sequence:
5'-CCGG-CGAGATATTCATCGTACATTT-CTCGAG-AAATGTACGATGAATATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATATTCATCGTACATTTCTCGAGAAATGTACGATGAATATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATATTCATCGTACATTTCTCGAGAAATGTACGATGAATATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000289641