Construct: shRNA TRCN0000049513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019893.1-413s1c1
DNA Barcode:
GCATCGACATAGGCATGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASAH2 (56624)
Gene Description:
N-acylsphingosine amidohydrolase 2
Transcript:
RefSeq NM_019893.1 (NON-CURRENT)
Match location:
Position 413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGACATAGGCATGGTAT
Hairpin Sequence:
5'-CCGG-GCATCGACATAGGCATGGTAT-CTCGAG-ATACCATGCCTATGTCGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGACATAGGCATGGTATCTCGAGATACCATGCCTATGTCGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGACATAGGCATGGTATCTCGAGATACCATGCCTATGTCGATGC-3'

Other clones with same target sequence:

(none)