Construct: shRNA TRCN0000050625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003069.2-1031s1c1
DNA Barcode:
GCTCACAGAATAAAGAATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCA1 (6594)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Transcript:
RefSeq NM_003069.2 (NON-CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACAGAATAAAGAATGAA
Hairpin Sequence:
5'-CCGG-GCTCACAGAATAAAGAATGAA-CTCGAG-TTCATTCTTTATTCTGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACAGAATAAAGAATGAACTCGAGTTCATTCTTTATTCTGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACAGAATAAAGAATGAACTCGAGTTCATTCTTTATTCTGTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000299496