Construct: shRNA TRCN0000053404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018921.2-29s1c1
DNA Barcode:
GCGGAAGATTAGTCCTGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGA9 (56107)
Gene Description:
protocadherin gamma subfamily A, 9
Transcript:
RefSeq NM_018921.2 (CURRENT)
Match location:
Position 29 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAAGATTAGTCCTGCTAT
Hairpin Sequence:
5'-CCGG-GCGGAAGATTAGTCCTGCTAT-CTCGAG-ATAGCAGGACTAATCTTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAAGATTAGTCCTGCTATCTCGAGATAGCAGGACTAATCTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAAGATTAGTCCTGCTATCTCGAGATAGCAGGACTAATCTTCCGC-3'

Other clones with same target sequence:

(none)