Construct: shRNA TRCN0000053813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018915.2-3885s1c1
DNA Barcode:
CCAGCCATAAACCAATAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGA2 (56113)
Gene Description:
protocadherin gamma subfamily A, 2
Transcript:
RefSeq NM_018915.2 (NON-CURRENT)
Match location:
Position 3885 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCATAAACCAATAACTA
Hairpin Sequence:
5'-CCGG-CCAGCCATAAACCAATAACTA-CTCGAG-TAGTTATTGGTTTATGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCATAAACCAATAACTACTCGAGTAGTTATTGGTTTATGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCATAAACCAATAACTACTCGAGTAGTTATTGGTTTATGGCTGG-3'

Other clones with same target sequence:

(none)