Construct: shRNA TRCN0000054786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023794.2-226s1c1
DNA Barcode:
AGGAAGTTTGTGGACACAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Etv5 (104156)
Gene Description:
ets variant 5
Transcript:
RefSeq NM_023794.2 (CURRENT)
Match location:
Position 226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAGTTTGTGGACACAGAT
Hairpin Sequence:
5'-CCGG-AGGAAGTTTGTGGACACAGAT-CTCGAG-ATCTGTGTCCACAAACTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAGTTTGTGGACACAGATCTCGAGATCTGTGTCCACAAACTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAGTTTGTGGACACAGATCTCGAGATCTGTGTCCACAAACTTCCT-3'

Other clones with same target sequence:

(none)