Construct: shRNA TRCN0000056454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018923.2-358s1c1
DNA Barcode:
GCAGTAATTGTGCAGGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGB2 (56103)
Gene Description:
protocadherin gamma subfamily B, 2
Transcript:
RefSeq NM_018923.2 (CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAATTGTGCAGGATATA
Hairpin Sequence:
5'-CCGG-GCAGTAATTGTGCAGGATATA-CTCGAG-TATATCCTGCACAATTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAATTGTGCAGGATATACTCGAGTATATCCTGCACAATTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAATTGTGCAGGATATACTCGAGTATATCCTGCACAATTACTGC-3'

Other clones with same target sequence:

(none)