Construct: shRNA TRCN0000060329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021270.2-508s1c1
DNA Barcode:
CCTCCGGATTTGATGCACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LAIR2 (3904)
Gene Description:
leukocyte associated immunoglobulin like receptor 2
Transcript:
RefSeq NM_021270.2 (NON-CURRENT)
Match location:
Position 508 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCGGATTTGATGCACCAT
Hairpin Sequence:
5'-CCGG-CCTCCGGATTTGATGCACCAT-CTCGAG-ATGGTGCATCAAATCCGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCGGATTTGATGCACCATCTCGAGATGGTGCATCAAATCCGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCGGATTTGATGCACCATCTCGAGATGGTGCATCAAATCCGGAGG-3'

Other clones with same target sequence:

(none)