Construct: shRNA TRCN0000063563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206997.1-316s1c1
DNA Barcode:
GCCGCTTCTACTACTTCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR152 (390212)
Gene Description:
G protein-coupled receptor 152
Transcript:
RefSeq NM_206997.1 (CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCTTCTACTACTTCCTAT
Hairpin Sequence:
5'-CCGG-GCCGCTTCTACTACTTCCTAT-CTCGAG-ATAGGAAGTAGTAGAAGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCTTCTACTACTTCCTATCTCGAGATAGGAAGTAGTAGAAGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCTTCTACTACTTCCTATCTCGAGATAGGAAGTAGTAGAAGCGGC-3'

Other clones with same target sequence:

(none)