Construct: shRNA TRCN0000065214

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005829.3-144s1c1
DNA Barcode:
GCGGGATGACAACATCTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3S2 (10239)
Gene Description:
adaptor related protein complex 3 subunit sigma 2
Transcript:
RefSeq NM_005829.3 (NON-CURRENT)
Match location:
Position 144 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065214)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGATGACAACATCTGTAA
Hairpin Sequence:
5'-CCGG-GCGGGATGACAACATCTGTAA-CTCGAG-TTACAGATGTTGTCATCCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGATGACAACATCTGTAACTCGAGTTACAGATGTTGTCATCCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGATGACAACATCTGTAACTCGAGTTACAGATGTTGTCATCCCGC-3'

Other clones with same target sequence:

  1. TRCN0000300223