Construct: shRNA TRCN0000066120
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_009439.1-1028s1c1
- DNA Barcode:
- CCTCTATTACACAGGGCGAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Psmd3 (22123)
- Gene Description:
- proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
- Transcript:
- RefSeq NM_009439.1 (CURRENT)
- Match location:
- Position 1028 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000066120)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCTATTACACAGGGCGAAT
- Hairpin Sequence:
-
5'-CCGG-CCTCTATTACACAGGGCGAAT-CTCGAG-ATTCGCCCTGTGTAATAGAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCTATTACACAGGGCGAATCTCGAGATTCGCCCTGTGTAATAGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCTATTACACAGGGCGAATCTCGAGATTCGCCCTGTGTAATAGAGG-3'
Other clones with same target sequence:
(none)
