Construct: shRNA TRCN0000067475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013485.1-228s1c1
DNA Barcode:
CCTTGCCTCAAACAAAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C9 (12279)
Gene Description:
complement component 9
Transcript:
RefSeq NM_013485.1 (CURRENT)
Match location:
Position 228 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCCTCAAACAAAGGTTT
Hairpin Sequence:
5'-CCGG-CCTTGCCTCAAACAAAGGTTT-CTCGAG-AAACCTTTGTTTGAGGCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCCTCAAACAAAGGTTTCTCGAGAAACCTTTGTTTGAGGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCCTCAAACAAAGGTTTCTCGAGAAACCTTTGTTTGAGGCAAGG-3'

Other clones with same target sequence:

(none)