Construct: shRNA TRCN0000068578
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175194.2-411s1c1
- DNA Barcode:
- GCAATGATGATTCGGATCTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc25a16 (73132)
- Gene Description:
- solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16
- Transcript:
- RefSeq NM_175194.2 (CURRENT)
- Match location:
- Position 411 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000068578)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAATGATGATTCGGATCTTT
- Hairpin Sequence:
-
5'-CCGG-GCAATGATGATTCGGATCTTT-CTCGAG-AAAGATCCGAATCATCATTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAATGATGATTCGGATCTTTCTCGAGAAAGATCCGAATCATCATTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAATGATGATTCGGATCTTTCTCGAGAAAGATCCGAATCATCATTGC-3'
Other clones with same target sequence:
(none)
