Construct: shRNA TRCN0000068897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028231.2-790s1c1
DNA Barcode:
GAGACCATGAAGATCAATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnmb2 (72413)
Gene Description:
potassium large conductance calcium-activated channel, subfamily M, beta member 2
Transcript:
RefSeq NM_028231.2 (CURRENT)
Match location:
Position 790 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCATGAAGATCAATCAA
Hairpin Sequence:
5'-CCGG-GAGACCATGAAGATCAATCAA-CTCGAG-TTGATTGATCTTCATGGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCATGAAGATCAATCAACTCGAGTTGATTGATCTTCATGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCATGAAGATCAATCAACTCGAGTTGATTGATCTTCATGGTCTC-3'

Other clones with same target sequence:

(none)