Construct: shRNA TRCN0000069611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145156.1-700s1c1
DNA Barcode:
GCAGAGGATGCAGATGTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a28 (246696)
Gene Description:
solute carrier family 25, member 28
Transcript:
RefSeq NM_145156.1 (CURRENT)
Match location:
Position 700 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGGATGCAGATGTACAA
Hairpin Sequence:
5'-CCGG-GCAGAGGATGCAGATGTACAA-CTCGAG-TTGTACATCTGCATCCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGGATGCAGATGTACAACTCGAGTTGTACATCTGCATCCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGGATGCAGATGTACAACTCGAGTTGTACATCTGCATCCTCTGC-3'

Other clones with same target sequence:

(none)