Construct: shRNA TRCN0000071211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007415.2-3021s1c1
DNA Barcode:
GAGTACATTGTCTACGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parp1 (11545)
Gene Description:
poly (ADP-ribose) polymerase family, member 1
Transcript:
RefSeq NM_007415.2 (CURRENT)
Match location:
Position 3021 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTACATTGTCTACGACATT
Hairpin Sequence:
5'-CCGG-GAGTACATTGTCTACGACATT-CTCGAG-AATGTCGTAGACAATGTACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTACATTGTCTACGACATTCTCGAGAATGTCGTAGACAATGTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTACATTGTCTACGACATTCTCGAGAATGTCGTAGACAATGTACTC-3'

Other clones with same target sequence:

  1. TRCN0000353959