Construct: shRNA TRCN0000071943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145459.3-2210s1c1
DNA Barcode:
CGGAATTAACGTGGACGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp503 (218820)
Gene Description:
zinc finger protein 503
Transcript:
RefSeq NM_145459.3 (CURRENT)
Match location:
Position 2210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATTAACGTGGACGTGAA
Hairpin Sequence:
5'-CCGG-CGGAATTAACGTGGACGTGAA-CTCGAG-TTCACGTCCACGTTAATTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATTAACGTGGACGTGAACTCGAGTTCACGTCCACGTTAATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATTAACGTGGACGTGAACTCGAGTTCACGTCCACGTTAATTCCG-3'

Other clones with same target sequence:

(none)